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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
07/07/1993 |
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Data da última atualização: |
02/05/2023 |
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Autoria: |
ROBINSON, S. P.; LOVEYS, B. R.; CHACKO, E. K. |
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Afiliação: |
Division of Horticulture,CSIRO, GPO Box 350, Adelaide, SA5001, Australia.; Division, fo Horticulture, CSIRO, Tropical Ecosystems Laboratory, PMB 44, Winnellie, NT 0821, Australia. |
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Título: |
Polyphenol oxidase enzymes in the sap and skin of mango fruit |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Australian Journal of Plant Physiology, v.20, n.1, p.99-107,1993 |
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ISSN: |
0310-7841 |
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Idioma: |
Inglês |
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Conteúdo: |
Severe occurs in mango fruit of the cultivar Kensington when sap contacts the fruit, resulting in browning and then blackening of the skin. Both the sap and skin of mango fruit contained considerable polyphenol oxidase (PPO) activity. The sap enzyme was not activated by SDS, was inhibited by hexadecyltrimethylammonium bromide, and was active with both para-and ortho-diphenol substrates. The skin enzyme was activated by SDS, was inhibited by salicylhydroxamic acid and polyvinylpyrrolidone, and active only with ortho-diphenol substrates. These properties suggest that the sap PPO is a laccase-type enzyme (EC 1.10.3.2) whereas the skin contains the common catwchol oxidase-type PPO (EC 1.10.3.1). The skin enzyme had a temperature optimum at 30 degrees C but the enzyme had maximum PPO activity at 75 degrees C. Both enzymes were relatively thermostable, requiring more than 15 min at 80 degrees C for 50% loss of activity. It is concluded that browning of mango skin induced by the sap is predominantly catalysed by PPO in the skin and this is unlikely to be prevented by heat treatment of the fruit. |
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Palavras-Chave: |
Cultivar. |
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Thesagro: |
Fungo; Temperatura. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01640naa a2200193 a 4500
001 1632646
005 2023-05-02
008 1993 bl uuuu u00u1 u #d
022 $a0310-7841
100 1 $aROBINSON, S. P.
245 $aPolyphenol oxidase enzymes in the sap and skin of mango fruit$h[electronic resource]
260 $c1993
520 $aSevere occurs in mango fruit of the cultivar Kensington when sap contacts the fruit, resulting in browning and then blackening of the skin. Both the sap and skin of mango fruit contained considerable polyphenol oxidase (PPO) activity. The sap enzyme was not activated by SDS, was inhibited by hexadecyltrimethylammonium bromide, and was active with both para-and ortho-diphenol substrates. The skin enzyme was activated by SDS, was inhibited by salicylhydroxamic acid and polyvinylpyrrolidone, and active only with ortho-diphenol substrates. These properties suggest that the sap PPO is a laccase-type enzyme (EC 1.10.3.2) whereas the skin contains the common catwchol oxidase-type PPO (EC 1.10.3.1). The skin enzyme had a temperature optimum at 30 degrees C but the enzyme had maximum PPO activity at 75 degrees C. Both enzymes were relatively thermostable, requiring more than 15 min at 80 degrees C for 50% loss of activity. It is concluded that browning of mango skin induced by the sap is predominantly catalysed by PPO in the skin and this is unlikely to be prevented by heat treatment of the fruit.
650 $aFungo
650 $aTemperatura
653 $aCultivar
700 1 $aLOVEYS, B. R.
700 1 $aCHACKO, E. K.
773 $tAustralian Journal of Plant Physiology$gv.20, n.1, p.99-107,1993
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
19/08/1993 |
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Data da última atualização: |
13/06/2011 |
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Autoria: |
BRAGA SOBRINHO, R.; FREIRE, E. C. |
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Afiliação: |
ELEUSIO CURVÊLO FREIRE, Pesquisador - Embrapa Algodão. |
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Título: |
Distribuição dos algodoeiros no nordeste do Brasil. |
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Ano de publicação: |
1982 |
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Fonte/Imprenta: |
Campina Grande: EMBRAPA-CNPA, 1982. |
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Páginas: |
38p. |
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Idioma: |
Português |
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Conteúdo: |
Zoneamento do nordeste para a cultura algodoeira. |
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Palavras-Chave: |
Brasil; Ceara; Distribuição; Distribution; Natural distribution; Nordeste; Paraiba; Pernambuco; Regiao nordeste; Rio Grande do Norte; Zoneamento. |
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Thesagro: |
Algodão; Distribuição Geográfica; Gossypium Hirsutum. |
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Thesaurus NAL: |
cotton. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/33345/1/DISTRIBUICAO-DOS-ALGODOEIROS.pdf
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Marc: |
LEADER 00834nam a2200313 a 4500
001 1262592
005 2011-06-13
008 1982 bl uuuu u0uu1 u #d
100 1 $aBRAGA SOBRINHO, R.
245 $aDistribuição dos algodoeiros no nordeste do Brasil.
260 $aCampina Grande: EMBRAPA-CNPA$c1982
300 $a38p.
520 $aZoneamento do nordeste para a cultura algodoeira.
650 $acotton
650 $aAlgodão
650 $aDistribuição Geográfica
650 $aGossypium Hirsutum
653 $aBrasil
653 $aCeara
653 $aDistribuição
653 $aDistribution
653 $aNatural distribution
653 $aNordeste
653 $aParaiba
653 $aPernambuco
653 $aRegiao nordeste
653 $aRio Grande do Norte
653 $aZoneamento
700 1 $aFREIRE, E. C.
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